DMRpred - Disordered Moonlighting Region predictor

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DMRpred webserver

This server is designed for the prediction of disordered moonlighting regions (DMRs). The server generates numeric score for each residue in the input protein sequence that quantifies putative propensity to constitue a disordered moonlighting region. Larger value of propensity denotes higher likelihood to form the disordered moonlighting residue. It also provides putative binary annotations - a given residue is predicted either as part of a disordered moonlighting region or not involved in the disorder moonlighting functions. This binary annotation is established using a threshold of 0.761 on the propensity score, which results in the false positive rate of 5%.

Please follow the three steps below to make predictions:

1. Upload a file with protein sequences, or paste them into text area

Server accepts up to 50 (FASTA formated) protein sequences with minimum length of 21 residues. Either upload a file or enter each protein in a new line in the following text field (see Help for details):

2. Provide your e-mail address (required)

Please provide your e-mail address to be notified when results are ready.

3. Predict:

Click button to launch prediction.

Help

DMRpred accepts either single or multiple protein sequences. The input is limited to 50 protein sequences, and each squence should be at least 21 residues long. The user should submit the protein sequence(s) in FASTA format.

The format of the input file is as follows (Here is an example of input file where DMRpred normally takes less than one minute to predict a protein with average size of 500 AAs.):

  • Line1: >protein ID
  • Line2: protein sequence (1-letter amino acid encoding)

Materials

    Explanation of TRAINING and TEST dataset
  • Line 1: >protein ID
  • Line 2: protein sequence (1-letter amino acid encoding)
  • Line 3: annotations of disordered moonlighting regions, where '1' denotes a disordered moonlighting residue, '0' denotes a structured residue or a residue that is disordered but not moonlighting, 'x' denotes a residue that lacks annotation.

References

Upon the usage the users are requested to use the following citation:

Acknowledgments

We acknowledge with thanks the following software used as a part of this server:

  • HHblits - Protein sequence searching by HMM-HMM alignment
  • IUPred - Prediction of Intrinsically Unstructured Proteins
  • ASAquick - Prediction of protein accessible surface area