The server is designed for the prediction of protein-binding residues. The server generates numeric score for each residue in the input protein sequence that quantifies putative propensity to bind proteins. Larger values of propensity denote higher likelihood to bind.

Please follow the three steps below to make predictions:

1. Copy and paste protein sequence(s) into text area

The Server accepts up to 10 FASTA formatted protein sequences with minimum length of 21 residues. Please enter protein sequence(s) in the following text field.

2. Provide your email address (optional)

Please enter your email address in the following text area or leave it blank. A link to prediction results will be sent to your email address once they are ready.

3. Predict

Click button to launch prediction.

Help

The webserver accepts either single or multiple protein sequence(s). The input is limited to 10 protein sequences. The user should submit the protein sequence(s) in the FASTA format.

The format of the input file is as follows:

  • Line1: >protein ID
  • Line2: protein sequence (1-letter amino acid encoding)

Each protein requires two lines and multiple proteins should be placed in consecutive lines.

Here is an example input file.

Materials

    Datasets and Supporting Information
  • Training dataset: Click here to download
  • Test dataset: Click here to download
  • Human proteome predictions: Click here to download
  • The format of the above files is explained here

Acknowledgments

We acknowledge with thanks the following software used as a part of this server:

  • ANCHOR - Prediction of protein binding regions in disordered proteins
  • ASAquick - Fast neural network-based predictor of solvent accessibility
  • HHBlits - Algorithm for homology detection by iterative HMM-HMM comparison
  • PSIPRED - Prediction of secondary structure