DEPICTER2 : DisorderEd PredictIon CenTER2

The server is designed to predict intrinsically disordered regions, disordered linkers and disordered binding regions from protein sequences.

Please follow the four steps below to make predictions: (click on this link for tutorials)

1. Enter input into the text area

Users can provide either only comma-separated Uniprot accession number(s) or only (FASTA formatted) protein sequence(s) as input. Each input protein sequence should have minimum length of 26 residues and maximum length of 5000 residues. The server accepts up to 25 sequences when the default/fast methods are selected. It accepts up to two sequences if DisoLipPred is selected. See Help for details concerning format.

2. Provide your e-mail address (optional)

Please enter your email address in the following text area. A link to predictions will be sent to this email address once they are ready. The results will be also available in the browser window.

3. Select methods that will be included in the prediction

	Fast Methods (default)
	A. Disorder prediction (flDPnn)
	B. Disordered linker prediction (DFLpred)
	C. MoRF prediction (MoRFchibiLight)
	D. Disordered protein-binding prediction (ANCHOR2)
	E. Disordered DNA-binding prediction (DisoRDPbind)
	F. Disordered RNA-binding prediction (DisoRDPbind)
	Slow Method
	G. Disordered lipid-binding prediction (DisoLipPred)

4. Predict

Click RUN to launch prediction

Help

The server accepts up to 25 protein sequences when fast methods are selected (methods A to F) and up to two sequences if DisoLipPred (method G) is chosen.

Submit either only Uniprot accession number(s) or only protein sequence(s).

Uniprot accession numbers should be comma-separated, as follows

P04637,P54252

Protein sequences should be in FASTA format, as follows

Line1: >Protein ID
Line2: Protein sequence (one-letter amino acid encoding)

Sequences less than 26 amino acids long cannot be predicted because these are peptides and the predictors cannot process them.

Sequences longer than 5000 amino acids can be partitioned into 5000 amino acids long segments. Each segment should be predicted individually and the prediction should be combined together.

The server typically takes less than one minute to produce predictions using fast methods (on average 10 secs per method) for an average size protein sequence.

Reference

Upon the usage of this server, users are requested to use the following citation:

Basu, S, Gsponer J, Kurgan L. DEPICTER2: A comprehensive webserver for intrinsic disorder and disorder function prediction. Nucleic Acids Research, gkad30, 2023.

Acknowledgments

We acknowledge with thanks the following software used as a part of this server:

flDPnn - Prediction of intrinsic disorder
MoRFchibiLight - Prediction of Molecular Recognition Feature (MoRF)
ANCHOR2 - Prediction of disordered protein-binding region
DisoRDPbind - Prediction of disordered RNA- and DNA-binding region
DisoLipPred - Prediction of disordered lipid-binding region
DFLpred - Prediction of disordered linker region