hybridPBRpred webserver

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This webserver is designed for the prediction of protein-binding residues. It generates numeric score for each residue in the input protein sequence that quantifies putative propensity for protein interaction. Larger values of propensity denote higher likelihood to interact.

HybridPBRpred combines prediction of protein-binding residues generated by SCRIBER (predictor that was trained using structures of protein-protein complexes) and DisoRDPbind (predictor that was trained using disordered protein-binding residues), with the underlying goal to produce complete set of the protein-binding residues.

Please follow the three steps below to make predictions:

1. Copy and paste protein sequence into text area

The webserver accepts a FASTA formatted protein sequence with minimum length of 21 residues. Please enter the protein sequence in the following text field.

2. Provide your email address (optional)

Please enter your email address in the following text area or leave it blank. A link to prediction results will be sent to your email address once they are ready.

3. Predict

Click the button to launch prediction.

Help

The webserver accepts a single protein sequence as the input. The user should submit the protein sequence in the FASTA format.

The format of the input file is as follows:

  • Line1: >protein ID
  • Line2: protein sequence (1-letter amino acid encoding)

Here is an example input file.

Materials

    Benchmark dataset and Supplement
  • Test dataset: Click here to download (The format is explained here)
  • Supplement: Click here to download

References

Upon the usage the users are requested to use the following citation:

Acknowledgments

We acknowledge with thanks the following software used as a part of this server:

  • SCRIBER - Prediction of protein binding regions in structured proteins
  • DisoRDPbind - Prediction of protein binding regions in disordered proteins
  • ANCHOR - Prediction of protein binding regions in disordered proteins
  • ASAquick - Fast neural network-based predictor of solvent accessibility
  • HHBlits - Algorithm for homology detection by iterative HMM-HMM comparison
  • PSIPRED - Prediction of secondary structure
  • BLAST - Alignment to proteins with annotated functions
  • IUPred - Prediction of Intrinsically Unstructured Proteins
  • PSIPRED - Prediction of secondary structure
  • SEG - Application for the prediction of low complexity regions (LCRs)